Abstract Agouti expressed in human adipocytes enhances lipogenesis and inhibits lipolysis through increasing intracellular Ca 2+. This factor stimulates fat accumulation through a paracrine/autocrine manner. However, agouti gene expression has not been clarified in bovine adipocytes. In the present study, it is found that agouti mRNA was expressed in adipocytes prepared from bovine adipose tissue using reverse transcription polymerase chain reaction. This is thought to be the first report where agouti gene is expressed in adipocyte of wild-type animal except for humans. This result suggests the possibility that agouti regulates adipocyte function in cattle.