1. Introduction.
2. Defining stress in aquatic animals.
3. Pain in aquatic animals.
4. The nature of anaesthesia, sedation and analgesia.
5. The features of anaesthetic agents and their legal use.
6. Anaesthesia and Legislation.
7. Factors affecting the response of aquatic ectotherms to anaesthesia.
8. Anaesthesia of fish. I. Inhalation anaesthesia.
9. Anaesthesia of fish. II. Inhalation anaesthesia using gases.
10. Anaesthesia of fish. III. Parenteral and oral anaesthesia.
11. Anaesthesia of fish. IV. Non chemical methods.
12. Anaesthesia of Aquatic Invertebrates.
13. Anaesthesia of Amphibians and Reptiles.
14. Transportation and anaesthesia.
15. Concluding remarks