Overview Introduction to Transgenic Animal Technology Introduction Historical Background Applications and Overview of Text References Transgenic Animal Production Focusing on the Mouse Model DNA Microinjection and Transgenic Animal Production Introduction General Methods Summary Acknowledgments References Appendix Factors Affecting Transgenic Animal Production Introduction Getting Started Troubleshooting Transgenic Phenomenology Summary Acknowledgments References Gene Targeting in Embryonic Stem Cells History and Methodology Stem Cells of the Embryo Gene Targeting in Embryonic Stem Cells Methods Acknowledgments References Gene Targeting in Embryonic Stem Cells Conditional Technologies Introduction Conditional Modeling: A Brief History Cre–loxP System Review, Development and Testing Cre–loxP and Transgenic Models Methods Summary Acknowledgments References Retrovirus-Mediated Gene Transfer Introduction and Discussion Methods Acknowledgment References Nuclear Transfer Technologies Introduction and Discussion Materials and Equipment Preparation of Microtools Nuclear Transfer of Preimplantation Embryos Nuclear Transfer of Embryonic Stem Cells and Somatic Cells Acknowledgment References Production of Transgenic Laboratory and Domestic Animal Species Production of Transgenic Rats Introduction Importance of Rats Contributions from Transgenic Rats Production Conclusions References Production of Transgenic Rabbits Applications of Transgenic Rabbits Manipulation of Rabbit Embryos for Production of Transgenics Materials and Methods References Production of Transgenic Fish Introduction Discussion Methods of Gene Transfer Establishing and Maintaining Transgenic Lineages Care and Containment of Transgenic Fish Future of the Transgenic Fish Acknowledgments References Production of Transgenic Poultry Introduction and Discussion Methods Summary References Production of Transgenic Swine by DNA Microinjection Introduction and Discussion Methods Alternative Methods for the Production of Transgenic Swine Summary References Production of Transgenic Ruminants by DNA Microinjection Introduction Production of Zygotes Suitable for Microinjection Microinjection of Ruminant Zygotes Transfer of Microinjected Embryos Areas of Application and Perspectives Acknowledgments References Production of Transgenic Nonhuman Primates Introduction Methods Summary Acknowledgments References Nuclear Transfer Technology in Cattle, Sheep, and Swine Introduction General Methods Summary Acknowledgments References Molecular Biology, Analyses, and Enabling Technologies Vector Design for Transgene Expression Introduction Transcribed Region Promoter Region Boundary Regions Conclusions and Perspective Acknowledgment References Analysis of Transgene Integration Introduction Detection of the Transgene Parameters for Evaluating a Transgene Analysis of the Transgene Establishing a Transgenic Line Derivation of Homozygous Mice Summary References PCR Optimization for Detection of Transgene Integration Introduction Discussion Summary References Analysis of Transgene Expression Introduction Analyses of Steady-State Levels of Transgenic RNA Transcripts Analyses of Steady-State Levels of Transgenic Protein Products Conclusions References Assisted Reproductive Technologies and Embryo Culture Methods for Farm Animals Introduction Assisted Reproductive Technologies Development of New Assisted Reproductive Technologies Summary References Databases, Internet Resources, and Genetic Nomenclature Introduction Transgenic and Targeted Mutation Databases and Electronic Resources Standardized Nomenclature for Transgenic and Targeted Mutation Animals References Additional Mouse Databases and Internet Resources Rat Databases and Internet Resources Swine Databases and Internet Resources Sheep Databases and Internet Resources Cattle Databases and Internet Resources Poultry Databases and Internet Resources Fish Databases and Internet Resources