The Golden Retriever Muscular Dystrophy dog lacks dystrophin and shares pathological and clinical similarities with the Duchenne patients. The model, increasingly used in pre-clinical trials, needs to be further characterized with non-invasive functional and imaging techniques. We defined NMR imaging indices of canine dystrophic muscle. Five GRMD and 5 control dogs were examined at the age of 2, 4 and 6 months with a 3 T NMR scanner. Anterior and posterior paws of the dogs were imaged with T1 and T2-weighted sequences, both being repeated with and without fat suppression. In the anterior paws, Extensor carpi radialis and flexor carpi ulnaris were studied. In the posterior paws Anterior tibialis, extensor digitorum, peroneus longus, and gastrocnemius were evaluated. Indices were calculated as follows: T2w/T1w signal ratio (SR = T2w Signal x T1w ref gain)/ T1w Signal x T2w ref gain), T1w and T2w heterogeneity (H=radical dot(SD2-SD2noise/0,655) ). A three-way analysis of variance was performed. T2w/T1w SR, H2, H1 were found significantly increased in dystrophic muscles at 3 T, at all ages. These quantitative indices differentiate dystrophic from normal muscles and might be proposed as non-invasive evaluation tools of therapeutic trials.