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Analysis by high throughput sequencing of Specific Pathogen Free eggs.

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Article
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Gagnieur, L. ; Cheval, Justine ; Cochet, M. ; Bréard, Emmanuel ; Gratigny, M. ; Hebert, Charles ; Muth, E. ; Viarouge, Cyril ; Dumarest, M. ; Coulpier, Muriel ; Eloit, Marc

BIOLOGICALS

1Institut Pasteur, Laboratory of Pathogen Discovery, Department of Virology, 28 rue du Docteur Roux, F-75724 Paris, France. 2 PathoQuest, Bâtiment François Jacob, 25 rue du Dr Roux, 75015, Paris, France. 3 Ecole Nationale Vétérinaire d'Alfort, UMR 1161 Virologie ENVA, INRA, ANSES, 7 Avenue Général de Gaulle, F-94704 Maisons Alfort, France. 4 Institut Pasteur, Laboratory of Pathogen Discovery, Department of Virology, 28 rue du Docteur Roux, F-75724 Paris, France; PathoQuest, Bâtiment François Jacob, 25 rue du Dr Roux, 75015, Paris, France; Ecole Nationale Vétérinaire d'Alfort, UMR 1161 Virologie ENVA, INRA, ANSES, 7 Avenue Général de Gaulle, F-94704 Maisons Alfort, France. Electronic address: marc.eloit@pasteur.fr.

2014

Article

Abstract Specific Pathogen Free (SPF) embryonated eggs are used for the production of many veterinary and human vaccines. We have used High Throughput Sequencing to screen allantoic fluids and embryos for the presence of encapsidated viral genomes and viral transcripts, respectively. SPF eggs from two different producers were tested. We evidenced sequences corresponding to known endogenous retroviruses and sequences of Avian Leukosis Virus, but no sequence that might suggest a productive infection of eggs with a virus even distant from known viruses. Our results strongly suggest that SPF eggs such as those used for this study represent a safe substrate for the production of vaccines.
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